Journal: Biomedicines

Article Title: Time-Dependent Effects of Cisplatin on Autophagy Gene Expression in Bladder Cancer Cells

doi: 10.3390/biomedicines14051006

Figure Lengend Snippet: Time-dependent cytotoxic effect of cisplatin and determination of IC 50 values in T24 bladder cancer cells. Cell viability was evaluated at 24 and 48 h time points following exposure to increasing concentrations of cisplatin (0–40 µM). The resulting dose–response curves demonstrate that cisplatin decreases cell viability in a dose- and time-dependent manner. The horizontal dashed line represents 50% cell viability, while the vertical dashed lines indicate the corresponding IC 50 values at the respective time points. The lower IC 50 values observed at 48 h of exposure indicate the increasing cytotoxic effect of cisplatin over time.

Article Snippet: Human bladder cancer cell lines T24 and 5637 (ATCC, Manassas, VA, USA) were utilized in this study.

Techniques:

Journal: Biomedicines

Article Title: Time-Dependent Effects of Cisplatin on Autophagy Gene Expression in Bladder Cancer Cells

doi: 10.3390/biomedicines14051006

Figure Lengend Snippet: Time-dependent cytotoxic effect of cisplatin and determination of IC 50 values in 5637 bladder cancer cells. Cells were exposed to varying concentrations of cisplatin for 24 and 48 h, and cell viability was assessed. The dose–response curves indicate a more pronounced sensitivity to cisplatin in 5637 cells. The horizontal dashed line represents 50% cell viability, while the vertical dashed lines indicate the calculated IC 50 values. Lower IC 50 values compared to T24 cells demonstrate that this cell line is more sensitive.

Article Snippet: Human bladder cancer cell lines T24 and 5637 (ATCC, Manassas, VA, USA) were utilized in this study.

Techniques:

Journal: Biomedicines

Article Title: Time-Dependent Effects of Cisplatin on Autophagy Gene Expression in Bladder Cancer Cells

doi: 10.3390/biomedicines14051006

Figure Lengend Snippet: Time-dependent heatmap of autophagy-related gene expression in T24 and 5637 bladder cancer cells following cisplatin treatment. Relative expression levels (2 −ΔΔCt ) are shown for genes involved in autophagy initiation, elongation, selective autophagy, and lysosomal function across 0, 6, 24, and 48 h.

Article Snippet: Human bladder cancer cell lines T24 and 5637 (ATCC, Manassas, VA, USA) were utilized in this study.

Techniques: Gene Expression, Expressing

Journal: Biomedicines

Article Title: Time-Dependent Effects of Cisplatin on Autophagy Gene Expression in Bladder Cancer Cells

doi: 10.3390/biomedicines14051006

Figure Lengend Snippet: Time-dependent expression profiles of autophagy-related genes in T24 and 5637 bladder cancer cell lines following cisplatin treatment. Relative mRNA expression levels of genes involved in autophagy initiation ( ULK1 , BECN1 ), elongation ( ATG5 , ATG7 , LC3B ), selective autophagy ( p62/SQSTM1 ), and lysosomal regulation (LAMP1 , TFEB ) were evaluated at 0, 6, 24, and 48 h post-treatment. Gene expression levels were calculated using the 2 −ΔΔCt method and are presented as fold changes relative to the control group (0 h). Blue bars represent the T24 cell line, and orange bars represent the 5637 cell line. Data are expressed as mean ± SEM of at least three independent experiments. * p < 0.05, ** p < 0.01 indicating statistical significance between the T24 and 5637 cell lines at the corresponding time points.

Article Snippet: Human bladder cancer cell lines T24 and 5637 (ATCC, Manassas, VA, USA) were utilized in this study.

Techniques: Expressing, Gene Expression, Control